Describe and discuss the diverse ways in which the development of Essay

Describe and discuss the diverse ways in which the development of second generation sequencing technologies has extended the fie - Essay Example On the same note, the four components related to barcoding include specimens, laboratory analysis, database and data analysis (CBOL Plant Working Group 2009, p.12794). Since early 90s, DNA sequencing has involved the use of capillary-based and semi-automated techniques related to Sanger biochemistry. The process of DNA sequencing then involved two approaches that include shotgun sequencing and PCR amplification. Shotgun sequencing involves a process of cloning DNA that through a random fragmentation and transformed into high-copy-number plasmid that is used for changing Escherichia coli. PCR amplification, on the other hand involves a process of targeted resequencing where primers are used to flank the target. Following three decades of improvements, the Sanger biochemistry, is now applied to obtain read lengths that average 1000 bp and accuracies in regard to per base raw that average 99.999%(Hutchison 2007, pp.6227-6237). However, the introduction of second generation sequencing te chniques continues to expand the field of DNA barcoding beyond the Sanger sequencing technique. The second-generation technologies have contributed to alternative DNA barcoding strategies and can be grouped in a number of categories. This includes sequencing using hybridization, cyclic-array sequencing, microelectrophoretic techniques and observation of single molecules in real-time (Healy 2007; Shendure 2005; Soni & Meller 2007). Second generation technologies as used in the field of barcoding implies to the different types of sequencing that have been introduced recently, in a commercial product and includes 454 sequencing, Solexa technology, Heliscope technology of single molecule sequencer, the Polonator and the SoLiD platform. These products have improved the diversity of sequencing, and have helped in the application of alternative protocols for purposes of generating jumping libraries related to mate-paired tags that contain controlled distance distributions. Further, these n ew technologies through various approaches, permits the production of amplicons that are clonally clustered, and acts as sequencing features. A common feature among the second-generation technologies in DNA barcoding is that, PCR amplicons emanating from various single library molecules can be spatially clustered on a single site within a planar substrate or on micron-scale bead’s surface. The sequencing process has further improved because of the introduction of alternating cycles related to enzymes-based biochemistry and data acquisition that is based on imaging (Mitra et al. 2003, pp. 55-62). In essence, the benefits of the second-generation technologies in comparison to the Sanger technique in diversifying DNA barcoding includes, the introduction of in vitro construction related to sequencing library. This is followed by cloning amplifications that produce sequencing features and circumvent numerous bottlenecks considered affecting parallelism related to sequencing consid ered as conventional. Second generation technologies compared to Sanger sequencing, have an advantage in terms of introducing array-based sequencing. Because of the existence of an array-based sequencing, the process of DNA barcoding is able to realize a considerable degree of parallelism compared to capillary-based sequencing.

Shopping Essay Example | Topics and Well Written Essays - 2000 words

Shopping - Essay Example In addition, such clothes keep on changing over the time, steadily and with considerable thought going into the design of new concepts. The western style military uniforms adopted by many modern military regimes brings in view the fact that even these garments are directly influenced by fashion, where shopping has absolutely no concern. The shopping can be restricted to a particular age where as fashion cannot be restricted, we cannot expect a child to go out for shopping and buying heavy stuff and clothing material but we can for sure expect a child to go out to visit a barber and get and stylish cut in the form of service which is also reflecting fashion, perhaps more natural one. But if we get more honest here then we should relate fashion to a particular gender, as we all know that women have a greater part in fashion to play where as men have little. Sawchuk once quoted in 1987 that, "Women's love of clothes, cosmetics, jewellery, their obsession with style and fashion, reinforces the myth that we are narcissistic and materialistic. In turn, this reinforces capitalism, which depends upon this obsession with our bodies for the marketing of new products"(Craik 1994: 47). Lets emphasize on another point which is perhaps more well defined in explaining that fashion is another major aim behind shopping. Just imagine what happens when anyone goes out for shopping, lets put some light behind the entire process starting from the designer or maker to the customer. Lets consider the example of any modern rising brand such as Christian Dior, the company previously was not allocating the need in the men's segment and was manufacturing perfumes and few other apparel for women, the... This essay declares that the shopping can be restricted to a particular age where as fashion cannot be restricted, we cannot expect a child to go out for shopping and buying heavy stuff and clothing material but we can for sure expect a child to go out to visit a barber and get and stylish cut in the form of service which is also reflecting fashion, perhaps more natural one. But if we get more honest here then we should relate fashion to a particular gender, as we all know that women have a greater part in fashion to play where as men have little. This paper makes a conclusion that there is another interesting point about fashion and that is at times we cannot simply relate fashion with shopping, simply by acknowledging that not all clothing is fashion, all clothing systems have at least a distant relationship with fashion systems and stylistic conventions. Military, religious and legal clothing can be related to earlier dress codes where associations of tradition, authority, order and distinctiveness are purposely called upon. Such clothes keep on changing over the time, steadily and with considerable thought going into the design of new concepts. The western style military uniforms adopted by many modern military regimes brings in view the fact that even these garments are directly influenced by fashion, where shopping has absolutely no concern. Shopping is not just related to the usual buying but it is much related to fashion.